Feature

Fluorescence lifetime imaging microscopy

By , and 2009-07-01T00:00:00

Green fluorescent protein

Scientists are using this non-invasive technique to cast light on the workings of living cells to learn more about the molecular mechanisms involved in cancer, allergies and immune responses

Fluorescence lifetime imagingmicrosopy (FLIM) is a non-invasive technique in which an image of a fluorescent probe or marker can be obtained in a cell without compromising or damaging the cell. The combination of scanning laser beams and powerful computers has advanced the field rapidly over the past 20 years. Fluorescence characteristics such as wavelength, lifetime and polarisation can now be recorded relatively easily and quickly. 

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