This book teaches about modern chemical techniques without heavy emphasis on maths or physics. It includes descriptions of instruments and their applications.
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Chromatography
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Level
Category
Specification
- Republic of Ireland
- England
- A/AS level
- AQA Chemistry
- Organic chemistry
- Amino acids, proteins and DNA
- Chromatography
- Chromatography
- Chromatography can be used to separate and identify the components in a mixture.
- Types of chromatography include: thin-layer chromatography (TLC) – a plate is coated with a solid and a solvent moves up the plate.
- Column chromatography (CC) – a column is packed with a solid and a solvent moves down the column.
- Gas chromatography (GC) – a column is packed with a solid or with a solid coated by a liquid, and a gas is passed through the column under pressure at high temperature.
- Separation depends on the balance between solubility in the moving phase and retention by the stationary phase.
- Retention times and Rf values are used to identify different substances.
- The use of mass spectrometry to analyse the components separated by GC.
- Students should be able to: calculate Rf values from a chromatogram.
- Compare retention times and Rf values with standards to identify different substances.
- Chromatography
- Organic chemistry
- Edexcel Chemistry
- Topic 19: Modern analytical techniques
- Topic 19C: Chromatography
- 6. know that chromatography separates components of a mixture between a mobile phase and a stationary phase
- 7. be able to calculate Rf values from one-way chromatograms
- 8 i. know that high performance liquid chromatography, HPLC, and gas chromatography, GC: are types of column chromatography
- 8 ii. separate substances because of different retention times in the column
- 8 iii. may be used in conjunction with mass spectrometry, in applications such as forensics or drugs testing in sport
- Topic 19C: Chromatography
- Topic 19: Modern analytical techniques
- OCR Chemistry A
- AQA Chemistry
- A/AS level
- Scotland
- Higher
- SQA Chemistry
- 3. Chemistry in society
- (e) Chemical analysis
- (i) Chromatography
- Chromatography is a technique used to separate the components present within a mixture. Chromatography separates substances by making use of differences in their polarity or molecular size.
- The details of any specific chromatographic method or experiment are not required. Depending on the type of chromatography used, the identity of a component can be indicated either by the distance it has travelled, or by the time it has taken to travel…
- (i) Chromatography
- (e) Chemical analysis
- 3. Chemistry in society
- SQA Chemistry
- Advanced Higher
- SQA Chemistry
- Researching Chemistry
- Practical skills and techniques
- Thin-layer chromatography
- Candidates should be familiar with thin-layer chromatography experimental procedure.
- Rf values can be calculated and under similar conditions a compound will always have the same Rf value within experimental error.
- Since a pure substance will show up as only one spot on the chromatogram, TLC can be used to assess the purity of a product prepared in the lab.
- Thin-layer chromatography
- Practical skills and techniques
- Researching Chemistry
- SQA Chemistry
- Higher
- Wales
- Northern Ireland
- GCSE
- CCEA Double award science
- Unit C1: Structures, Trends, Chemical Reactions, Quantitative Chemistry and Analysis
- 1.9 Chemical analysis
- 1.9.6 describe paper chromatography as the separation of mixtures of soluble substances by running a solvent (mobile phase) through the mixture on the paper (stationary phase), which causes the substances to move at different rates over the paper;
- 1.9.7 interpret a paper chromatogram including calculating Rf values;
- 1.9 Chemical analysis
- Unit C1: Structures, Trends, Chemical Reactions, Quantitative Chemistry and Analysis
- CCEA Chemistry
- Unit 1: Structures, Trends, Chemical Reactions, Quantitative Chemistry and Analysis
- 1.9 Chemical analysis
- 1.9.6 describe paper chromatography as the separation of mixtures of soluble substances by running a solvent (mobile phase) through the mixture on the paper (stationary phase), which causes the substances to move at different rates over the paper;
- 1.9.7 interpret a paper chromatogram including calculating Rf values;
- 1.9 Chemical analysis
- Unit 1: Structures, Trends, Chemical Reactions, Quantitative Chemistry and Analysis
- CCEA Double award science
- A/AS level
- GCSE
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