In this experiment you will see how methanol and ethanol behave differently when treated with iodine and sodium hydroxide solutions.

Class practical

This experiment shows how methanol and ethanol behave differently when treated with iodine solution and sodium hydroxide solution. Ethanol reacts to form a yellow solid, tri-iodo-methane, often called iodoform.

Lesson organisation

This experiment takes about 20 minutes. It is eminently suitable for students who have the iodoform reaction in their specification. For others, it is a good observational exercise and an extension for more able students.

Both methanol and ethanol (and other alcohols and organic liquids) can be stored in plastic pipettes, from which they can be easily dispensed. The main difficulty for students arises from the fact that if the pipettes are squeezed too hard, the alcohols come out of the pipette in a stream (because of their low surface tension). Students must handle the pipettes very carefully, and some practice will be required before proceeding with this experiment.

Apparatus Chemicals

Eye protection: goggles

Each group of students will need:

Test-tubes, 2

Liquids or solutions in plastic dropping pipettes (one of each):


Ethanol (HIGHLY FLAMMABLE) or Industrial denatured alcohol, IDA (HIGHLY FLAMMABLE, HARMFUL)

Sodium hydroxide, 1 M (CORROSIVE)

Iodine solution, 0.5 M (dissolved in potassium iodide solution 0.2 M)

Refer to Health & Safety and Technical notes section below for additional information.

Health & Safety and Technical notes

Read our standard health & safety guidance

Wear goggles throughout. 

Methanol, CH3OH(l), (HIGHLY FLAMMABLE, TOXIC) - see CLEAPSS Hazcard.


Sodium hydroxide, NaOH(aq), (CORROSIVE at concentration used) - see CLEAPSS Hazcard and CLEAPSS Recipe Book.

Iodine solution, I2(aq), dissolved in potassium iodide solution, KI(aq), - see CLEAPSS Hazcard and CLEAPSS Recipe Book. 

Product: Tri-iodo-methane, CHI3(s), (HARMFUL) - see CLEAPSS Hazcard. 


a Practice (over a sink) producing single drops of ethanol from the pipette.

b Add 10 drops of methanol to one test-tube.

c Add 10 drops of ethanol to the other test-tube.

d Add 25 drops of iodine solution to each alcohol.

e Add 10 drops of sodium hydroxide solution to each alcohol.

f  Gently swirl the test-tubes a few times. The dark colour of the iodine should
start to fade.

g After 2 min carefully observe the two test-tubes. What differences do you notice?

Teaching notes

The solution in the ethanol test-tube should go cloudy and then a yellow precipitate of tri-iodo-methane (iodoform) should be seen. This has a distinct ‘antiseptic’ smell. The methanol test-tube should remain clear.

The iodoform reaction is given by compounds with a methyl group next to a carbonyl group. Secondary alcohols with a CH3 on the carbon carrying the OH (eg propan-2-ol) that can be oxidised to carbonyl compounds of this type, also give a positive iodoform test. (NB carboxylic acids do not)

Ethanol is the only primary alcohol which will give the reaction and ethanal the only aldehyde. An explanation of the reaction is:

The negative charge on the carbon in the I3C ion is stabilised by the three electronegative iodine atoms.

Health & Safety checked, July 2016 


This Practical Chemistry resource was developed by the Nuffield Foundation and the Royal Society of Chemistry.

© Nuffield Foundation and the Royal Society of Chemistry


Chemguide  - gives more details of the iodoform reaction

Page last updated July 2016