Brady’s test for aldehydes and ketones

Test tubes containing yellow, red and orange substances formed by the combination of two solutions

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Identify aldehydes and ketones using Brady’s reagent (2,4-dinitrophenylhydrazine) in this microscale experiment. Includes kit list and safety instructions.

In this practical, students add various liquid aldehydes and ketones to 2,4-dinitrophenylhydrazine solution in a well-plate or spotting tile to form solid derivatives. They then do the same test with methanol and ethanol, showing that the reaction does not occur with alcohols.

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